stemdiff definitive endoderm kit tesr-e8 optimized Search Results


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ATCC assays fibroblast basal medium atcc pcs 201 030 fibroblast growth kit low serum atcc pcs 201 041 stemdiff definitive endoderm kit
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stemdiff definitive endoderm kit tesr-e8 optimized  (STEMCELL Technologies Inc)
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Stemdiff Definitive Endoderm Kit Tesr E8 Optimized, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stemdiff cardiomyocyte differentiation kit  (STEMCELL Technologies Inc)
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Identification of induced pluripotent stem cell based <t>cardiomyocytes</t> (A) Immunofluorescence staining of Oct4 (a)(d), TRA-1-60 (a)(d), SOX2 (b)(e) and SSEA4 (b)(e) in iPSC of ARVC or control groups. Positive staining of Oct4 (red) and SOX2 (red) within the cell nuclei, and positive staining of TRA-1-60 (green) and SSEA4 (green) on the cell membrane. Immunofluorescence staining of TNNT-2 (c)(f) and NKX2.5 (c)(f) in iPSC-CMs of ARVC or control group. Positive staining of NKX2.5 (red) within cell nuclei, and positive staining of TNNT-2 (green) on the cell membrane. Cell nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI, blue). Scale bars of 50μm (images on the upper left); and 20μm (images on the upper right). (B) Images of Oil Red O staining images of CMs from ARVC and the control groups (a)(d). Most ARVC-iPSC-CMs were positivity stained for lipid droplets (red) within cells, whereas only a small number of control cells were stained positive for lipids. Scale bar: 100 μm. TEM images of iPSC-CMs containing lipid droplets in cell cytoplasm (b and e, 10000×) and the ultrastructure of CMs (c and f, 30000×). Small-scale lipid droplets (L) observed close to the nucleus (N) in iPSC-CMs from a control subject (b) and the ARVC patient (e). iPSC-CMs from the ARVC patient appear to possess a larger number of lipid droplets in comparison to control cells. Myofibrils (my) are organized in different sarcomeric structures according to the description of Z-bands (Z). Glycogen masses (gly) can be seen in the cytoplasm. N, nucleus.
Stemdiff Cardiomyocyte Differentiation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stemdiff smadi neural induction kit  (STEMCELL Technologies Inc)
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Identification of induced pluripotent stem cell based <t>cardiomyocytes</t> (A) Immunofluorescence staining of Oct4 (a)(d), TRA-1-60 (a)(d), SOX2 (b)(e) and SSEA4 (b)(e) in iPSC of ARVC or control groups. Positive staining of Oct4 (red) and SOX2 (red) within the cell nuclei, and positive staining of TRA-1-60 (green) and SSEA4 (green) on the cell membrane. Immunofluorescence staining of TNNT-2 (c)(f) and NKX2.5 (c)(f) in iPSC-CMs of ARVC or control group. Positive staining of NKX2.5 (red) within cell nuclei, and positive staining of TNNT-2 (green) on the cell membrane. Cell nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI, blue). Scale bars of 50μm (images on the upper left); and 20μm (images on the upper right). (B) Images of Oil Red O staining images of CMs from ARVC and the control groups (a)(d). Most ARVC-iPSC-CMs were positivity stained for lipid droplets (red) within cells, whereas only a small number of control cells were stained positive for lipids. Scale bar: 100 μm. TEM images of iPSC-CMs containing lipid droplets in cell cytoplasm (b and e, 10000×) and the ultrastructure of CMs (c and f, 30000×). Small-scale lipid droplets (L) observed close to the nucleus (N) in iPSC-CMs from a control subject (b) and the ARVC patient (e). iPSC-CMs from the ARVC patient appear to possess a larger number of lipid droplets in comparison to control cells. Myofibrils (my) are organized in different sarcomeric structures according to the description of Z-bands (Z). Glycogen masses (gly) can be seen in the cytoplasm. N, nucleus.
Stemdiff Smadi Neural Induction Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gentle cell dissociation reagent  (STEMCELL Technologies Inc)
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Identification of induced pluripotent stem cell based <t>cardiomyocytes</t> (A) Immunofluorescence staining of Oct4 (a)(d), TRA-1-60 (a)(d), SOX2 (b)(e) and SSEA4 (b)(e) in iPSC of ARVC or control groups. Positive staining of Oct4 (red) and SOX2 (red) within the cell nuclei, and positive staining of TRA-1-60 (green) and SSEA4 (green) on the cell membrane. Immunofluorescence staining of TNNT-2 (c)(f) and NKX2.5 (c)(f) in iPSC-CMs of ARVC or control group. Positive staining of NKX2.5 (red) within cell nuclei, and positive staining of TNNT-2 (green) on the cell membrane. Cell nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI, blue). Scale bars of 50μm (images on the upper left); and 20μm (images on the upper right). (B) Images of Oil Red O staining images of CMs from ARVC and the control groups (a)(d). Most ARVC-iPSC-CMs were positivity stained for lipid droplets (red) within cells, whereas only a small number of control cells were stained positive for lipids. Scale bar: 100 μm. TEM images of iPSC-CMs containing lipid droplets in cell cytoplasm (b and e, 10000×) and the ultrastructure of CMs (c and f, 30000×). Small-scale lipid droplets (L) observed close to the nucleus (N) in iPSC-CMs from a control subject (b) and the ARVC patient (e). iPSC-CMs from the ARVC patient appear to possess a larger number of lipid droplets in comparison to control cells. Myofibrils (my) are organized in different sarcomeric structures according to the description of Z-bands (Z). Glycogen masses (gly) can be seen in the cytoplasm. N, nucleus.
Gentle Cell Dissociation Reagent, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il- 34  (PeproTech)
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Identification of induced pluripotent stem cell based <t>cardiomyocytes</t> (A) Immunofluorescence staining of Oct4 (a)(d), TRA-1-60 (a)(d), SOX2 (b)(e) and SSEA4 (b)(e) in iPSC of ARVC or control groups. Positive staining of Oct4 (red) and SOX2 (red) within the cell nuclei, and positive staining of TRA-1-60 (green) and SSEA4 (green) on the cell membrane. Immunofluorescence staining of TNNT-2 (c)(f) and NKX2.5 (c)(f) in iPSC-CMs of ARVC or control group. Positive staining of NKX2.5 (red) within cell nuclei, and positive staining of TNNT-2 (green) on the cell membrane. Cell nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI, blue). Scale bars of 50μm (images on the upper left); and 20μm (images on the upper right). (B) Images of Oil Red O staining images of CMs from ARVC and the control groups (a)(d). Most ARVC-iPSC-CMs were positivity stained for lipid droplets (red) within cells, whereas only a small number of control cells were stained positive for lipids. Scale bar: 100 μm. TEM images of iPSC-CMs containing lipid droplets in cell cytoplasm (b and e, 10000×) and the ultrastructure of CMs (c and f, 30000×). Small-scale lipid droplets (L) observed close to the nucleus (N) in iPSC-CMs from a control subject (b) and the ARVC patient (e). iPSC-CMs from the ARVC patient appear to possess a larger number of lipid droplets in comparison to control cells. Myofibrils (my) are organized in different sarcomeric structures according to the description of Z-bands (Z). Glycogen masses (gly) can be seen in the cytoplasm. N, nucleus.
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Image Search Results


Identification of induced pluripotent stem cell based cardiomyocytes (A) Immunofluorescence staining of Oct4 (a)(d), TRA-1-60 (a)(d), SOX2 (b)(e) and SSEA4 (b)(e) in iPSC of ARVC or control groups. Positive staining of Oct4 (red) and SOX2 (red) within the cell nuclei, and positive staining of TRA-1-60 (green) and SSEA4 (green) on the cell membrane. Immunofluorescence staining of TNNT-2 (c)(f) and NKX2.5 (c)(f) in iPSC-CMs of ARVC or control group. Positive staining of NKX2.5 (red) within cell nuclei, and positive staining of TNNT-2 (green) on the cell membrane. Cell nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI, blue). Scale bars of 50μm (images on the upper left); and 20μm (images on the upper right). (B) Images of Oil Red O staining images of CMs from ARVC and the control groups (a)(d). Most ARVC-iPSC-CMs were positivity stained for lipid droplets (red) within cells, whereas only a small number of control cells were stained positive for lipids. Scale bar: 100 μm. TEM images of iPSC-CMs containing lipid droplets in cell cytoplasm (b and e, 10000×) and the ultrastructure of CMs (c and f, 30000×). Small-scale lipid droplets (L) observed close to the nucleus (N) in iPSC-CMs from a control subject (b) and the ARVC patient (e). iPSC-CMs from the ARVC patient appear to possess a larger number of lipid droplets in comparison to control cells. Myofibrils (my) are organized in different sarcomeric structures according to the description of Z-bands (Z). Glycogen masses (gly) can be seen in the cytoplasm. N, nucleus.

Journal: Theranostics

Article Title: Intracellular calcium current disorder and disease phenotype in OBSCN mutant iPSC-based cardiomyocytes in arrhythmogenic right ventricular cardiomyopathy

doi: 10.7150/thno.45172

Figure Lengend Snippet: Identification of induced pluripotent stem cell based cardiomyocytes (A) Immunofluorescence staining of Oct4 (a)(d), TRA-1-60 (a)(d), SOX2 (b)(e) and SSEA4 (b)(e) in iPSC of ARVC or control groups. Positive staining of Oct4 (red) and SOX2 (red) within the cell nuclei, and positive staining of TRA-1-60 (green) and SSEA4 (green) on the cell membrane. Immunofluorescence staining of TNNT-2 (c)(f) and NKX2.5 (c)(f) in iPSC-CMs of ARVC or control group. Positive staining of NKX2.5 (red) within cell nuclei, and positive staining of TNNT-2 (green) on the cell membrane. Cell nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI, blue). Scale bars of 50μm (images on the upper left); and 20μm (images on the upper right). (B) Images of Oil Red O staining images of CMs from ARVC and the control groups (a)(d). Most ARVC-iPSC-CMs were positivity stained for lipid droplets (red) within cells, whereas only a small number of control cells were stained positive for lipids. Scale bar: 100 μm. TEM images of iPSC-CMs containing lipid droplets in cell cytoplasm (b and e, 10000×) and the ultrastructure of CMs (c and f, 30000×). Small-scale lipid droplets (L) observed close to the nucleus (N) in iPSC-CMs from a control subject (b) and the ARVC patient (e). iPSC-CMs from the ARVC patient appear to possess a larger number of lipid droplets in comparison to control cells. Myofibrils (my) are organized in different sarcomeric structures according to the description of Z-bands (Z). Glycogen masses (gly) can be seen in the cytoplasm. N, nucleus.

Article Snippet: The medium was changed to TeSR-E8 without ROCKi 1 day later and cells were further cultured for another 3 d. For cardiac differentiation, the STEMdiff Cardiomyocyte Differentiation Kit (05010, STEMCELL Technologies Inc, British Columbia, Canada) was applied to iPSC colonies according to the manufacturer's instructions.

Techniques: Immunofluorescence, Staining, Control, Membrane, Comparison